Masaka Regional Referral Hospital, commonly known as Masaka Hospital is a hospital in the city of Masaka, in south-central Uganda. It is the referral hospital for the districts of Kalangala District, Lyantonde, Masaka and Sembabule, Kalungu, Lwengo, Bukomansimbi and Rakai.

Masaka Hospital is a public hospital, funded by the Uganda Ministry of Health and general care in the hospital is free. It is one of the 13 Regional Referral Hospitals in Uganda. The hospital is designated as one of the 15 Internship Hospitals in Uganda where graduates of Ugandan medical schools can serve one year of internship under the supervision of qualified specialists and consultants. The official bed capacity of Masaka Hospital is quoted as 330, but often twice that number or more is accommodated. The hospital averages about 450 daily admissions.